DNA

Part:BBa_K2680554

Designed by: Stephanie Do   Group: iGEM18_William_and_Mary   (2018-10-16)


UNS 10 Sequence, from Torella et al., 2013

UNS 10 Sequence, from Torella et al., 2013

This is Unique Nucleotide Sequence 10, (UNS 10), from Torella et al., 2013. This UNS10 sequence is necessary for our 3G assembly, a method that combines Gibson and Golden Gate. For more information on 3G hybrid DNA assembly and 3G parts, see the William and Mary 3G Parts Page.


The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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